In 1202, an army led by the three vampire elders (Markus, Viktor, and Amelia) arrives at a Lycan-ravaged village. Viktor and Amelia capture their target, Markus' brother, William Corvinus, the first and most powerful werewolf. Despite Markus' defiance, Viktor orders that William be imprisoned in a secret location forever.
In the present day, vampire Selene takes Michael to a safe house so that she can confront the vampire regent Kraven; she knows that Kraven intends to kill Markus and plans to stop him. However, Markus has awakened before Kraven arrives. Markus kills Kraven and his men. Lorenz Macaro, an elderly man, sends in a team of "cleaners" to investigate the aftermath from the battle in the Lycans' lair. When Lorenz Macaro examines Viktor's corpse he finds a metal disc inside it which is the match to a pendant originally worn by Sonja. The other half of the pendant is now possessed by Selene and Michael after the deaths of Viktor and Lucian.
Markus tracks Selene and Michael down and attacks them, but they evade him and hide in a warehouse. There, Selene and Michael share their feelings and engage in sexual intercourse. Now knowing that the pendant is important to Markus, Michael and Selene set out to discover why Markus wants it. Selene recalls that she saw it as a child, but does not know its significance. They travel to the hideout of the exiled vampire historian Andreas Tanis who reveals that Markus was the first vampire; one of the three sons of Alexander Corvinus, the first immortal. Markus was bitten by a bat and became a vampire, while his twin brother William was bitten by a wolf and became a werewolf. The third son remained human and gave rise to a line of mortal descendants including Michael who became the first Lycan